We’ll apply our understanding of the Central Dogma of Molecular Biology to investigate the molecular basis of Sickle Cell Anemia.
I want you to consider three big ideas this week. One, if Red Blood Cells (RBCs) don’t have a nucleus, but Sickle Cell Anemia is a genetic disease, then where is the mutation that codes for the mutated hemoglobin found in the RBCs? Two, what is happening in the RBCs of an individual who is a carrier for Sickle Cell Anemia? Three, why do bacteria synthesize restriction enzymes?
Monday (4/26/10): Progress Reports, clean up lab
Tuesday (4/27/10): Set Up Restriction Digests. Cast gels if time permits
Wednesday (4/28/10): Run Digested Sickle Cell DNAs in 1/5% Agarose gel.
Thursday (4/29/10): Analyze Sickle Cell DNA electrophoresis, receive write up
Friday (4/30/10): Continue Sickle Cell Lab write up. Due Monday, 5/3/10.
Tags: central dogma, restriction enzymes, sickle cell anemia
