This is going to be a pretty laid back week as we only have four days of lab, and one of them has been taken over by Billy Shakespeare. As I like to tell Ms. Abernathy, my British heros run closer to Darwin, Watson and Crick (and Watson was an American). Anywho…we’re going to take it light this week, meaning we’re going to light up with Green…Flourescent Protein. Our goal is to prurify the protein and see if we can draw a picture with it. Consider it our own secret paint, visible only in UV light.
Tuesday (4/13/10): CALCULATING TRANSFORMATION EFFICIENCY. Set up overnights (O/N) of pGLO transformants in LB amp broth and LB amp/ara brothDrawing with transformed bacteria on LB/amp/ara plates
Wednesday (4/14/10): Students that are here, spin down O/N cultures, add lysozyme and freeze overnight
Thursday (4/15/10): Separate protein from cell lysate and purify using HIC columns.
Friday (4/16/10): pGLO transformation and GFP purification story boards.
Tags: GFP, pGLO, transformation
